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Description
Human LTbR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Lymphotoxin Beta Receptor (LTbR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Lymphotoxin Beta Receptor (LTbR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lymphotoxin Beta Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lymphotoxin beta receptor (LTbR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxins involved in apoptosis and cytokine release. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) receptor family. It is expressed on the surface of most cell types, including epithelial and myeloid cells, but not on T and B lymphocytes. LTbR not only helps trigger apoptosis but also leads to the release of the cytokine interleukin-8. Overexpression of LTbR in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 2410 reviews
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Product Reviews
★★★★★ 5
Works well!
Style: 16 oz
This stuff works well. I've only ever used it after my tires had been punctured and ran flat... I haven't had a flat this wouldn't seal... 3 uses so far all 3 have fixed flats and been many miles put on since the repairs. I start with as little as possible and if it doesn't seal I just add more, I think I've managed to use far less than recommended and successfully sealed punctures. I've only used it on fat tire e-bike tires. I'm pretty comfortable with the seal and I have no plans to replace the tubes, which saves me a fair amount of trouble. I will update if it springs any leaks without new punctures.
It's very easy to use and actually pretty clean especially compared to automotive type tire repair foams.
The first repair I used it for was a brand new bike that just wouldn't hold air in the rear, totally unsure what caused it to keep losing air but 2 or 3 small doses of slime fixed it.
The most recent slime repair, I had a wobbly bike ride which felt weird and I couldn't figure out why but a few hours after I had returned home both tires were totally flat. They both had a handful or more of some really heavy duty thorns stuck in the tire that likely barely punctured the tubes but the rear tire also had a roofing nail in it. I did not think the slime was going to fill that one, it was a pretty heavy leak once I removed the nail, but Slime did seal it and I've since ridden the bike twice and in the same rough conditions I normally would still holding strong at higher pressure than I normally ride, have yet to drop back down since refilling but I am hoping and expecting it won't be a problem.
Two thumbs up. I always keep a small tube in my bike bag and I am confident if I ever need it while I'm out and about it will be useful.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Who can live without slime great stuff and a good price. 👍
Style: 32 oz
If anyone has an outdoor anything that takes air. You should never be caught without this stuff. It seals your tires on almost anything and it lasts longer than you would expect. It’s easy to use and easy to clean up after using it. It will work well for anything that is supposed to hold air. Great product.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Title: Reliable, Easy-to-Use, and Worth Every Penny
Style: 32 oz
I’ve been using Slime tire sealant for my fat tire e-bike, and I can say with confidence that this product delivers exactly what it promises. The application is simple, the results are consistent, and the peace of mind it provides is invaluable. For anyone who rides regularly — whether for commuting, recreation, or deliveries — this is a product that pays for itself many times over.
The first thing I appreciate about Slime is how easy it is to install. The bottle is well-designed, the instructions are clear, and the process takes just a few minutes. You simply deflate the tire, remove the valve core, squeeze in the recommended amount, reinstall the valve core, inflate, and spin the wheel to distribute the sealant evenly. No special tools, no mess, and no guesswork — just a straightforward, user-friendly process.
Performance-wise, Slime does exactly what it’s supposed to do: seal punctures quickly and effectively. I’ve had nails, thorns, and sharp debris puncture my tires while riding, and in every case where the leak was within the tread area, Slime sealed it instantly. There’s something incredibly reassuring about hearing that faint hiss of air for a second, then watching it stop as the sealant does its job. It’s a real-time reminder that this product is actively protecting you from being stranded.
What sets Slime apart from other sealants I’ve tried is its durability. Once applied, it stays active inside the tube for months, ready to respond to new punctures as they happen. It doesn’t dry out quickly, it doesn’t clump up, and it doesn’t cause balance issues with the tires. For someone who rides almost daily, that reliability is a huge plus.
Another thing I respect about this product is that it’s non-toxic and environmentally friendly. If a bit of sealant escapes during a repair, I don’t have to worry about it harming the environment. It’s also water-soluble, making cleanup easy if I ever need to replace a tube or tire. That attention to safety and sustainability shows that the company isn’t just making a great product — they’re doing it responsibly.
The value for the price is excellent. One bottle goes a long way, and when you factor in the cost of replacing tubes, paying for roadside repairs, or even the inconvenience of being late because of a flat, Slime is an investment in both savings and peace of mind. For delivery riders like me, it’s especially valuable — every flat avoided means another day of work uninterrupted.
In short, Slime is one of those rare products that I can recommend without hesitation. It’s easy to use, effective, long-lasting, and backed by a company that clearly understands the needs of cyclists and e-bike riders. It has already saved me from countless headaches, and I plan to keep a bottle on hand at all times. If you ride regularly and haven’t tried it yet, you’re taking an unnecessary risk — this is one of the best preventative tools you can add to your maintenance kit.
Verdict: Five stars for performance, ease of use, and overall value. Slime is a must-have for any serious rider who values reliability and wants to keep rolling no matter what’s on the road.
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Reviewed in the United States on August 11, 2025
★★★★★ 4
Slime is good, pump is a disaster
Style: 1 gal, Style: 1 gal
The only reason I am giving this a 4 star(not zero) rating is the slime itself. The actual pump on the 1 gallon canister is awful. It makes so much mess, so I do not know whether I pumped the slime into the tubes or it all just spilled out of the base of the pump. The bottles worked great, but this time I needed to use more slime for a bunch of tires. What a slimy mess that was. Very poor pump design. Only after I spilled who knows how much of the slime, I finally washed it and was able to see the problem. The cap at the base of the pump got unscrewed and let all the slime lose. I screwed it back up and this should fix the mess next time I use it. Just a heads if someone encounters the same issue.
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Great product
Style: 16 oz
Worked as advertised. I would order again.
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Reviewed in the United States on May 29, 2026