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Description
Human SMURF1 (E3 Ubiquitin-protein Ligase Smurf1) ELISA KitThe Human (SMURF1) E3 Ubiquitin protein Ligase Smurf1 ELISA Kit measures E3 ubiquitin protein ligase SMURF1 in samples. The plate has been pre coated with Human SMURF1 antibody. SMURF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SMURF1 Antibody is added and binds to SMURF1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SMURF1 antibody. After incubation unbound
The Human (SMURF1) E3 Ubiquitin-protein Ligase Smurf1 ELISA Kit measures E3 ubiquitin-protein ligase SMURF1 in samples. The plate has been pre-coated with Human SMURF1 antibody. SMURF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SMURF1 Antibody is added and binds to SMURF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SMURF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SMURF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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